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阅读次数:1168 发布时间:2020/6/9 10:12:41
为了感谢和回馈广大客户的支持,我司特设专项奖学金计划,旨在建立产品应用数据库,为广大科研工作者提供产品使用的参考价值。我们期待与您携手共同努力,在生命科学的探索道路上,取得更加卓越辉煌的成就。
近日,曲阳师范大学牟老师在《Frontiers in Physiology》期刊里发表了SCA文献并引用我司产品“仓鼠褪黑素(MT)elisa试剂盒”,已获得我司奖学金300元,恭喜牟老师,感谢您的支持!欢迎新老客户使用我司试剂盒,并踊跃参加我司活动!
【文献题目】 Photoperiod affects Harderian gland morphology and secretion in female Cricetulus barabensis: Autophagy,apoptosis, and mitochondria
【产品原文引用】:
Sample PreparationHamsters were sacrificed by CO2 asphyxiation at 22:00 on the last day, after all hamsters had been in darkness for at least 2 h. Bloodsamples were collected immediately from the heart after sacrifice,stored at 4?C for 30 min, and then centrifuged at 3000 rpm for 15 min at 4?C. Serum MT levels were estimated using an enzyme-linked immunosorbent assay (Shanghai Hengyuan Biological Technology Co., Ltd., H-40277, Shanghai, China). The HGs were removed, with lengths and weights then recorded. The HGs from five hamsters were fixed by immersion and processed for light and electron microscopy. The right HGs were immersed in 10% buffered formalin for paraffin section embedding, andthe left HGs were immersed in glutaraldehyde-paraformaldehyde for transmission electron microscopy (TEM). The remaining HGs from 15 hamsters in each group were frozen in liquid nitrogen and stored in a refrigerator at ?80℃ for subsequent Western blotting, real-time polymerase chain reaction (RTPCR), and immunofluorescence histochemical analyses. In each experiment, unilateral HGs from 10 hamsters were used for quantification. All procedures were carried out in accordance with approved guidelines.
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