【文献标题】Inhibited auhagy impairs systemic nutrient metabolism in Nile tilapia
【作者】Si-Lan Han，Jing Wang，Yu-Xue Zhang，et.al
【作者单位】华东师范大学（East China Normal University）
【文献中引用产品】
【关键词】Auhagy，Metabolism，Homeostasis，Inflammation，Nile tilapia
【DOI】https://doi.org/10.1016/j.cbpa.2019.06.021
【影响因子(IF)】4.0
【出版期刊】《Comparative Biochemistry ａnd Physiology, Part A》
【产品原文引用】
At the end of trial, all fish were fasted overnight, six fish of each group were euthanized (MS-222 at 20 mg/L) ａnd sampled to collect tissues to measure the molecular, protein ａnd biochemical indexes.Hepatic triglyceride (TG), glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), ａnd serum TG, free fatty acid (FFA), ａnd glucose were assessed by commercial kits (Jiancheng Biotech Co. China).The serum insulin was detected by ELISA kits (Hengyuan Biotech Co.China). Briefly, the total lipid of the whole fish body, liver ａnd muscle was extracted by using chloroform/methanol (2:1, v/v) as previously described (Bligh ａnd Dyer, 1959). Briefly, the samples were homogenized in the mixed chloroform-methanol 2:1 (vol/vol), ａnd the samples were stored at 4 °C for 24-h extraction. Afterwards, the chloroform phase was carefully moved to a clean glass tube ａnd dried using nitrogen, ａnd the extracted total lipid was weighed ａnd recorded.Whole fish protein ａnd muscle protein were measured by Kjeltec? 8200(FOSS, Sweden).
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