【文献标题】Dehydroepiandrosterone reduces accumulation of lipid droplets in primary chicken hepatocytes by biotransformation mediated via the cAMP/PKA-ERK1/2 signaling pathway

【作者】Longlong Li, Chongyang Ge, Dian Wang, et.al

【作者单位】南京农业大学（Nanjing Agricultural University）

【文献中引用产品】

鸡脱氢表雄酮（DHEA）elisa试剂盒

【关键词】Dehydroepiandrosterone，Fat accumulation，Biotransformation，cAMP/PKA-ERK1/2 signaling pathway，Primary chicken hepatocytes

【DOI】https://doi.org/10.1016/j.bbalip.2018.03.007

【影响因子(IF)】5.03

【出版期刊】《BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS》

【产品原文引用】

Rabbit anti-17β-HSD ａnd anti-aromatase antibodies were purchased from Abcam (Abcam, USA). Rabbit anti-AMPKα, phospho-AMPKα,ACCα, phospho-ACCα, ERK1/2, phospho-ERK1/2 ａnd β-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Goat anti-rabbit IgG horseradish peroxidase conjugate was purchased from Boster (Wuhan, China). U0126-EtOH (phospho-ERK1/2 inhibitor),Forskolin (PKA activator), H89 (PKA inhibitor), Trilostane (3β-HSD inhibitor), Flutamide (androgen receptor [AR] antagonist), ａnd Fulvestrant (estrogen receptor [ER] antagonist) were purchased from Selleck Chemicals (Houston, TX, USA). 12-O-tetradecanoyl phorbol-13-acetate (TPA, an ERK activator) was purchased from ApexBio (Houston,USA). Protein assay kits were obtained from the Beyotime Biotechnology Institute (Shanghai, China). DHEA Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Hengyuan Biological Technology Co. (Shanghai, China). Testosterone ａnd estradiol radioimmunoassay (RIA) kits were purchased from the Beijing Beifang Biotechnology Institution (Beijing, China). DHEA, estradiol, or testosterone were dissolved in DMSO ａnd stored at 20℃. The working solutions of DHEA, estradiol, or testosterone containing of DMSO were diluted in M199 medium, ａnd the DMSO content in all working solutions did not exceed 0.1% by volume.

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