先恭贺东北大学武老师使用上海恒远生物自主品牌ELISA试剂盒在《ACS Nano》（美国化学学会）期刊上发表的SCI文献——Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases顺利通过，IF影响因子高达15.88分。同时恭贺武老师喜提上海恒远生物赠送的奖学金，正所谓双喜临门呀！

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【文献标题】Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases
【作者单位】东北大学（Northeastern University）
【文献中引用产品】
人CD63分子（CD63）ELISA试剂盒
【关键词】uEVs ,DNA machine, fluorescence detection ,machine learning, urinary diseases
【DOI】https://doi.org/10.1021/acsnano.1c06429
【影响因子(IF)】15.88
【出版期刊】《ACS Nano》
【产品原文引用】
Materials.
All the oligonucleotides were synthesized ａnd purified by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China), ａnd their sequences are listed in Table S1. All oligonucleotide stock solutions were prepared by dissolving DNA strands in Tris-HCl buffer (10 mmol L-1 Tris, 10 mmol L-1 MgCl2, pH 8.0) ａnd stored at -20 °C refrigerator before use. All the DNA strands were annealed at 95 °C for 5 min ａnd slowly cooled down to room temperature to obtain stable structures. 1-Ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC) ａnd N?hydroxysuccinimide (NHS) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Sodium hydroxide (NaOH), Na2HPO4·12H2O, NaH2PO4·2H2O, AgNO3 ａnd KCl were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). 2-(N Morpholino) ethanesulfonic acid (MES), Gel-Red, loading buffer ａnd 5×TBE buffer were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). BCA Protein Quantification Kit was provided by Beyotime Biotechnology (Shanghai, China). Dulbecco’s modified eagle medium (DMEM) was acquired from Aladdin Reagent Co. Ltd. (Shanghai, China). Carboxyl magnetic nanoparticles (COOH-MNPs, 200 nm) was purchased from PuriMag Biotech Co. Ltd. (Xiamen, China). Exo III, phi29 DNA polymerase were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). T4 DNA ligase ａnd deoxynucleotides (dNTPs) were purchased from Takara Biotechnology Co. (Dalian, China). Thioflavine T (ThT) was obtained from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). Anti?CD63 rabbit monoclonal antibody(ab193349) was purchased from Abcam Co. Ltd. (Shang, China). Anti-CD9 rabbit monoclonal antibody (20597-1-ap), anti-TSG101 rabbit monoclonal antibody (28283-1-AP) ａnd β-actin monoclonal antibody (66009-1-Ig) were purchased from Proteintech Group Co. Ltd. (Chicago, USA). ELISA (human CD63) kit was purchased from Shanghai Hengyuan Biological Technology (Shanghai, China). All chemicals were analytical grade ａnd directly used without further purification.

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